Influence on rat intestinal absorption of huanglian alkalis after match-pair of huanglian-wuzhuyu / 中国中药杂志

<p><b>OBJECTIVE</b>To research effect of Wuzhuyu on Huanglian intestinal absorption and to provide some basic principle of match-pair of huanglian-rougui.</p><p><b>METHOD</b>The rat everted gut sac method as well as non everted gut sac method was applied to investigate the influence of Wuzhuyu on Huanglian intestinal absorption.</p><p><b>RESULT</b>The content of each alkalis in Huanglian was decreasing with the comptibale ration increase, and the intestinal absorption had been improved in some degree. The intestinal absorption of Huanglian alkalis reached the highest level when match-pair ration of huang Lian-rougui was 6: 1.</p><p><b>CONCLUSION</b>The chemical reaction in the progress of extraction and the intestinal absorption of huanglian alkalis were the key factors of match-pair of huanglian-wuzhuyu.</p>

Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.</p><p><b>METHOD</b>HPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them.</p><p><b>RESULT</b>Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol x L(-1) HCl, and furthermore their remained percentage was up to 98% and 103%.</p><p><b>CONCLUSION</b>The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol x L(-1) HCl in the solution.</p>

Comparative investigation of the components in rats plasma before and after orally administrating Shuanghuanglian injection / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the main constituents absorbed in the blood after orally administrating Shuanghuanglian injection extracts of its composed crude drug and provide the foundation for optimizing the prescription of Shuanghuanglian powder for injection.</p><p><b>METHOD</b>Through analysing HPLC fingerprint established based on shuanghuanglian powder for injection and extracts of it composed crude drug, the active investigation were carried out among the peaks of control plasma and plasma containing drug in rats, shuanghuanglian powder for injection and extract of its composed crude drug. And the analysis was performed about relative content of constituents in each sample. Furthermore their original drugs and the metabolites in plasma containing drug were ascertained.</p><p><b>RESULT</b>Eight compounds were detected in plasma after oral administration of Shuanghuanglian, four of them were the original compounds contained in Shuanghuanglian powder for injection and three of them were relative metabolites. It has also found that contents of original compounds such as baicalin and chlorogenic acid fell off gradually, but their metabolites increased in rat plasma in vivo. With increasing time the main components of forsythia have not been detected obviously in rats plasma.</p><p><b>CONCLUSION</b>In some extent, the compounds absorbed into blood from Shuanghuanglian powder for injection and their metabolites are the effective constituents, and its plasma pharmacochemistry should be subject to illuminate the pharmacological effects and the active mechanism.</p>

Influence of match-pair of huanglian-rougui on rat intestinal absorption of total huanglian alkali / 中国中药杂志

<p><b>OBJECTIVE</b>To research the principle of match-pair of huanglian-rougui.</p><p><b>METHOD</b>A rat intestinal model in vitro had been applied to investigate the absorption and transport characteristics of total huanglian alkali across intestinal mucosa, as well as the influence of match-pair of huanglian-rougui on its intestinal absorption. The identification of alkalis in primitive solution, serosal solution and intestinal homogenate were analyzed by TLC.</p><p><b>RESULT</b>The transport and uptake characteristic of total alkali in huanglian solution was similar to berberine. The uptake and transport of total alkali across intestinal mucosa was concentration-and time-dependent. Transport directions had strong effects on the transport of total alkali. The amount of total alkali transport from serosal side to mucosal side was much more than that from mucosal side to serosal side. After matching with rougui as some ration, the intestinal absorption of total alkali increased significantly comparing with huanglian alone, and there was an optimal ration among match-pair of huanglian-rougui.</p><p><b>CONCLUSION</b>Chemical reaction in vitro and intestinal absorption were the key factors of match-pair of huanglian-rougui.</p>

Degradation of recombinant hirudin-2 in nasal mucosa of rabbits in vitro / 药学学报

<p><b>AIM</b>To investigate the degradation of recombinant hirudin-2 (rHV2) in nasal mucosa of rabbit.</p><p><b>METHODS</b>The specific and accurate HPLC method was developed for analyzing rHV2; The degrading ratios of rHV2 at different concentrations and at pH conditions in rabbit nasal mucosa homogenate were determined; The results in nasal mucosa homogenate were compared with that in small intestinal mucosa homogenate of rabbits. The stability of rHV2 in the enzyme extract of nasal mucosa surface and the effect of proteolysis inhibitor bacitracin on the degradation of rHV2 in nasal mucosa homogenate were also estimated.</p><p><b>RESULTS</b>The degradation of rHV2 in rabbit nasal mucosa homogenate showed concentration- and pH-dependence; rHV2 in nasal mucosa homogenate was more stable than that in intestinal mucosa homogenate. Also rHV2 was more stable in the enzyme extracts of nasal serosal surface than that of mucosa surface. Addition of bacitracin was able to inhibit the degradation to some degree.</p><p><b>CONCLUSION</b>Comparing with oral administration, rHV2 nasal delivery was a more tolerant route.</p>